Shotgun lipidomics and mass spectrometry imaging unveil diversity and dynamics in Gammarus fossarum lipid composition

•Baseline lipidome profiling of G. fossarum different sex and reproductive stages•Spatial localization lipids in gammarid tissue by mass spectrometry imaging•SIMS imaging guided discovery sulfate-based hepatopancreas epithelium•Disclosure a dynamic lipid composition maturing female oocytes Sentinel species are playing an indispensable role monitoring environmental pollution aquatic ecosystems. Many pollutants found water prove to be endocrine disrupting chemicals that could cause disruptions homeostasis species. A comprehensive the these is thus essential step toward understanding mechanism toxicity induced pollutants. Both spatial distribution freshwater crustacean Gammarus were extensively examined herein. The baseline gammarids stages was established high throughput shotgun lipidomics. Spatial mapping resolution led their accumulation mature oocytes. diverse uncovered, which deepens our biochemical changes during development serve as reference for future ecotoxicological studies. 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